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GrainGenes Marker Report: Bmag5a

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Locus	Bmag5a
Type	Microsatellite
Chromosome	1H
Map	Hordeum-Consensus2006-DArT-1H 59.4
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Map Data	Barley, Consensus 2006, DArT
Probe	Bmag5
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of Bmag5a in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmag0005 Bmag5b
Probe for Locus	Bmag5
Locus	Bmag0005 Bmag5a Bmag5b
Synonym	Other name Bmag0005
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AG)15 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.45
Type	PCR SSR
PCR primers	5' TCCATGATGATGTGTGCATAGA 3' 5' CGGATCCCAACAAACACAC 3'
SSR size	173
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare