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GrainGenes Marker Report: Bmag603

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Locus	Bmag603
Type	Microsatellite
Synonym	Synonym Bmag0603
Chromosome	3H
Map	Barley-consensus-2003-3H 43.5 Hordeum-Consensus2006-DArT-3H 69.3 Hv-Consensus2006-Marcel-3H 54.55
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT Barley, Consensus 2006, Marcel Barley, Steptoe x Morex, SSR
Probe	Bmag603
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of Bmag603 in the Barley, Consensus 2006 DArT maps = 2 Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Steptoe x Morex
Possible Orthologs	Bmag0603
Probe	Bmag603
Locus	Bmag0603 Bmag603
Synonym	Other name Bmag0603
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)24 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.78
Type	PCR SSR
PCR primers	5' ATACCATGATACATCACATCG 3' 5' GGGGGTATGTACGACTAACTA 3' ATACCATGATACATCACATCG GGGGGTATGTACGACTAACTA
SSR size	120
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare