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GrainGenes Marker Report: Bmag7

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Locus	Bmag7
Synonym	Synonym Bmag0007
Chromosome	7H
Map	Hv-Consensus2006-Marcel-7H 20.62
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Map Data	Barley, Consensus 2006, Marcel Barley, Igri x Franka, SSR
Probe	Bmag7
Remark	Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Igri x Franka
Possible Orthologs	Bmag0007
Probe	Bmag7
Locus	Bmag0007 Bmag7
Synonym	Other name Bmag0007
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)16(AC)16 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.76
Type	PCR SSR
PCR primers	5' TGAAGGAAGAATAAACAACCAACA 3' 5' TCCCCTATTATAGTGACGGTGTG 3'
SSR size	185
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare