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GrainGenes Marker Report: Bmag720

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Locus
Bmag720
Chromosome
2H
Map
Hv-Consensus2006-Marcel-2H72.46
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Map Data
Barley, Consensus 2006, Marcel
Barley, Steptoe x Morex, SSR
Probe
Bmag720
Remark
Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Steptoe x Morex
Possible Orthologs
Bmag0720



Probe
Bmag720
Locus
Bmag0720
Bmag720
Synonym
Other nameBmag0720
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (GT)6,(AG)49
Quality = 2: Single product, relatively strong stutter bands
Diversity Index (DI) = 0.84
Type
PCR
SSR
PCR primers
5' AAACCGTTTGTATAGCACG 3'
5' ATAAGTGAATGCTTCTGAGGA 3'
SSR size
171
Amplification Conditions
3 min 94C, 45 cycles of 1 min @94C, 1 min @55C, 2 min @72C, final extension of 10 min @72C. 25 microlitre reactions contained 125 nM of each primer.
Source Species
Hordeum vulgare