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GrainGenes Marker Report: Bmag742

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Locus	Bmag742
Type	Microsatellite
Chromosome	2H
Map	Hordeum-SxM-SSR-2H 86.5 Hv-Consensus2006-Marcel-2H 72.01
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, Marcel Barley, Steptoe x Morex, SSR
Probe	Bmag742
Reference	Li JZ et al. (2003) Development and genetic mapping of 127 new microsatellite markers in barley Theoretical and Applied Genetics 107:1021-1027.
Remark	Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Steptoe x Morex
Possible Orthologs	Bmag0742
Probe	Bmag742
Locus	Bmag0742 Bmag742
Synonym	Other name BMAG0742 Bmag0742
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (TC)29 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.35
Type	PCR SSR
PCR primers	5' AAACAGAGGGTTTTAGTAATGG 3' 5' AGTGAGATGGCAGTACATAGG 3'
SSR size	148
Amplification Conditions	3 min 94C, 45 cycles of 1 min @94C, 1 min @55C, 2 min @72C, final extension of 10 min @72C. 25 microlitre reactions contained 125 nM of each primer.
Source Species	Hordeum vulgare