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GrainGenes Marker Report: Bmag749

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Locus	Bmag749
Type	Microsatellite
Chromosome	2H
Map	Barley-consensus-2003-2H 141.1 Barley-ConsensusFHB-2H 146.73 Hordeum-Consensus2006-DArT-2H 157.4 Hordeum-SxM-SSR-2H 191.7 Hv-Consensus2006-Marcel-2H 147.93
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Map Data	Barley consensus 2003 Barley, Consensus 2006, DArT Barley, Consensus 2006, Marcel Barley, Consensus 2023, FHB Barley, Steptoe x Morex, SSR
Probe	Bmag749
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Li JZ et al. (2003) Development and genetic mapping of 127 new microsatellite markers in barley Theoretical and Applied Genetics 107:1021-1027.
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of Bmag749 in the Barley, Consensus 2006 DArT maps = 2 Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Steptoe x Morex; Cebada Capa x SusPtrit
Possible Orthologs	Bmag0749
Probe	Bmag749
Locus	Bmag0749 Bmag749
Synonym	Other name Bmag0749
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)11 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.64
Type	PCR SSR
PCR primers	5' CGGATTCTTGAGTAGTCTCTG 3' 5' GATCTGTTTTTGTAGAACATGC 3'
SSR size	166
Amplification Conditions	3 min 94C, 45 cycles of 1 min @94C, 1 min @55C, 2 min @72C, final extension of 10 min @72C. 25 microlitre reactions contained 125 nM of each primer.
Source Species	Hordeum vulgare