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GrainGenes Marker Report: Bmag812

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Locus	Bmag812
Type	Microsatellite
Chromosome	5H
Map	Hordeum-SxM-SSR-5H 113.9 Hv-Consensus2006-Marcel-5H 90.32
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, Marcel Barley, Steptoe x Morex, SSR
Probe	Bmag812
Reference	Li JZ et al. (2003) Development and genetic mapping of 127 new microsatellite markers in barley Theoretical and Applied Genetics 107:1021-1027.
Remark	Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Steptoe x Morex
Possible Orthologs	Bmag0812
Probe	Bmag812
Locus	Bmag0812 Bmag812
Synonym	See also Bmag0812
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (CT)26 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.79
Type	PCR SSR
PCR primers	5' ATAGTTCTTTCAGGACCAATG 3' 5' GTCATATGGATCTCCAAAGAG 3'
SSR size	157
Amplification Conditions	3 min 94C, 45 cycles of 1 min @94C, 1 min @55C, 2 min @72C, final extension of 10 min @72C. 25 microlitre reactions contained 125 nM of each primer.
Source Species	Hordeum vulgare