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GrainGenes Marker Report: Bmag813

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Locus	Bmag813
Type	Microsatellite
Chromosome	2H
Map	Hordeum-Consensus2006-DArT-2H 79.6
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, DArT
Probe	Bmag813
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of Bmag813 in the Barley, Consensus 2006 DArT maps = 1
Possible Orthologs	Bmag0813
Probe	Bmag813
Locus	Bmag813
Synonym	See also Bmag0813
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (CT)6,(CT)7,(TATC)17 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.74
Type	PCR SSR
PCR primers	5' ATGGGTCCATAGCAATAGATA 3' 5' GGAAGAATCACATCAAATAGG 3'
SSR size	197
Amplification Conditions	3 min 94C, 45 cycles of 1 min @94C, 1 min @55C, 2 min @72C, final extension of 10 min @72C. 25 microlitre reactions contained 125 nM of each primer.
Source Species	Hordeum vulgare