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GrainGenes Marker Report: Bmag853

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Locus	Bmag853
Type	Microsatellite
Chromosome	3H
Map	Hordeum-SxM-SSR-3H 202.5 Hv-Consensus2006-Marcel-3H 144.1
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, Marcel Barley, Steptoe x Morex, SSR
Probe	Bmag853
Reference	Li JZ et al. (2003) Development and genetic mapping of 127 new microsatellite markers in barley Theoretical and Applied Genetics 107:1021-1027.
Remark	Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Steptoe x Morex
Possible Orthologs	Bmag0853
Probe	Bmag853
Locus	Bmag0853 Bmag853
Synonym	See also Bmag0853
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (GA)15 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products Diversity Index (DI) = 0.64
Type	PCR SSR
PCR primers	5' ACAAGTATCCTGCAAACCTAA 3' 5' CGACCTTCTTAATGGTTAGTG 3'
SSR size	183
Amplification Conditions	3 min 94C, 45 cycles of 1 min @94C, 1 min @55C, 2 min @72C, final extension of 10 min @72C. 25 microlitre reactions contained 125 nM of each primer.
Source Species	Hordeum vulgare