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GrainGenes Marker Report: Bmag9b

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Locus	Bmag9b
Type	Microsatellite
Chromosome	6H
Map	Hordeum-Consensus2006-DArT-6H 68.5
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Map Data	Barley, Consensus 2006, DArT
Probe	Bmag9
Reference	Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
Remark	Number of populations contributing to the placement of Bmag9b in the Barley, Consensus 2006 DArT maps = 4
Possible Orthologs	Bmag0009 Bmag9 Bmag9a
Probe for Locus	Bmag9
Locus	Bmag0009 Bmag9 Bmag9a Bmag9b
Synonym	Other name Bmag0009
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AG)13 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.59
Type	PCR SSR
PCR primers	5' AAGTGAAGCAAGCAAACAAACA 3' 5' ATCCTTCCATATTTTGATTAGGCA 3'
SSR size	172
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare