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GrainGenes Marker Report: CFD49

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Probe	CFD49
Locus	Xcfd49-6D Xcfd49 cfd49-6D SrTA10187 cfd49
Reference	Olson EL et al. (2013) Introgression of stem rust resistance gene SrTA10187 and SrTA10171 from Aegilops tauschii to wheat Theoretical and Applied Genetics 126:2477-2484. Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stem Rust Resistance. SrTA10187 MAS Wheat. Marker Assisted Selection in Wheat.
Remarks	Quality Score = A Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)33 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' TGAGTTCTTCTGGTGAGGCA 3' Reverse 5' GAATCGGTTCACAAGGGAAA 3'
SSR size	214
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	6D
Source Species	Aegilops tauschii