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GrainGenes Marker Report: Dhn2B

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Locus	Dhn2B
Type	RFLP
Map	Hordeum-Bins-4H 40
	[ Show Nearby Loci ]
Map Data	Barley, BinMap 2005
Probe	Dhn2
Data Source	Kleinhofs, Andris
Remark	This marker is a Binlocus in the Hordeum-Bins maps, i.e. a marker mapped in other crosses and located to the Steptoe x Morex bin
Probe for Locus	Dhn2
Locus	Dhn2B Dhn2A Dhn2C Dhn2
Synonym	Other name B9 b9
Reference	Choi DW et al. (2000) Map locations of barley Dhn genes determined by gene-specific PCR Theoretical and Applied Genetics 101:350-354. Close TJ et al. (1989) A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Molecular Biology 13:95-108.
General Remarks	Size in Dicktoo (bp) = 1030 Size in Morex (bp) = 965 B9-dehydrin
Type	cDNA PCR STS
PCR primers	5' CCAGCCGACCAGGGACGACCACAA 3' TCCGTCGTCCCCTGCATA
Amplification Conditions	Annealing temp. = 59
Linkage Group	Group 7
Source Gene Class	14.4 kDa dehydrin
Source Gene	Dhn2 (Hordeum)
Source Species	Hordeum vulgare
Insert Size	0.683