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GrainGenes Marker Report: Dhn4a

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Locus	Dhn4a
Type	RFLP
Map	Hordeum-DxM-5H 158.9
	[ Show Nearby Loci ]
QTL with Locus Marker	QGpc.DiMo-5H.2
Map Data	Barley, Dicktoo x Morex
Probe	Dhn4
Probe for Locus	Dhn4
Locus	XDhn3,4 Xucr.Dhn3,4 Dhn4 Dhn4a
Synonym	Other name B18
Reference	Choi DW et al. (2000) Map locations of barley Dhn genes determined by gene-specific PCR Theoretical and Applied Genetics 101:350-354. Close TJ et al. (1989) A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Molecular Biology 13:95-108.
General Remarks	Barley dehydrin gene 4 Size in Dicktoo (bp) = 998 Size in Morex (bp) = 881 B18-dehydrin
Type	cDNA PCR STS
PCR primers	5' CGGCAGCGCAAGATGGAGTACCAG 3' CCCCTCCAACAGCCAAGTGAGCTA
Amplification Conditions	Annealing temp. = 64
Sequence	AF043090
Source Gene Class	22.6 kDa dehydrin
Source Gene	Dhn4 (Hordeum)
Source Species	Hordeum vulgare
Insert Size	1.049