GrainGenes Marker Report: EBmac0607
Locus
EBmac0607
Type
Microsatellite
Chromosome
2H
Map
Barley, B73xCPI-6H 47.8 Barley-ConsensusFHB-2H 73.03 Hordeum-Consensus2007-SSR-2H 67.01 Hordeum-LxHs-2H 50
[ Show Nearby Loci ]
Map Data
Barley, B73xCPI Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, LxHs
Species
Hordeum spontaneum Hordeum vulgare
Probe
EBmac0607 EBmac607
Reference
Remark
Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (TG)7,(TG)6(GA)10, (AG)6 Polymorphic Information Content (PIC) = 0.38 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs
EBmac607 EBmac607b
Probe for Locus
EBmac607
Locus
EBmac0607 EBmac607 EBmac607b
Synonym
Other name EBmac0607
Reference
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (TG)7,(TG)6(GA)10,(AG)6 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.38
Type
PCR SSR
PCR primers
5' GCGAACATTGTCATGTTAGTA 3'
5' AACCTTATGGATTTGGAGG 3'
SSR size
146
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare
GrainGenes Marker Report: EBmac0607
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