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GrainGenes Marker Report: EBmac0755

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Locus	EBmac0755
Type	Microsatellite
Chromosome	7H
Map	Barley, B73xCPI-7H 155.9 [ Show all 7 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	QHea.pil-7H.4 QHei.pil-7H.6 QYld.pil-7H.3
Map Data	Barley, AxHs/QTL [ Show all 7 ]
Species	Hordeum vulgare
Probe	EBmac0755 EBmac755
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Possible Orthologs	EBmac755
Probe for Locus	EBmac755
Locus	EBmac0755 EBmac755
Synonym	Other name EBmac0755
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)16 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.72
Type	PCR SSR
PCR primers	5' AGCCTTGTGTATCAGGACA 3' 5' CTGCTGGTGTTCTCTAAAAGT 3' AGCCTTGTGTATCAGGACA CTGCTGGTGTTCTCTAAAAGT
SSR size	143
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare