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GrainGenes Marker Report: EBmac713

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Locus	EBmac713
Type	Microsatellite
Synonym	Synonym EBmac0713
Map Data	Barley, Steptoe x Morex, SSR
Probe	EBmac713
Possible Orthologs	EBmac0713
Probe	EBmac713
Locus	EBmac0713 EBmac713
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (CA)6TA(CA)3(GA)2(CA)6TA(CA)17 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.9
Type	PCR SSR
PCR primers	5' GGTAAAACATTTCCCTCGT 3' 5' TAGAGATCACTCTCTTCTGTGC 3' GGTAAAACATTTCCCTCGT TAGAGATCACTCTCTTCTGTGC
SSR size	168
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare