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GrainGenes Marker Report: EBmag705

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Locus	EBmag705
Type	Microsatellite
Chromosome	3H
Map	Barley-consensus-2003-3H 109 Hv-Consensus2006-Marcel-3H 145.35
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Map Data	Barley consensus 2003 Barley, Consensus 2006, Marcel Barley, Steptoe x Morex, SSR
Probe	EBmag705
Data Source	Langridge, Peter J.
Remark	Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Steptoe x Morex
Possible Orthologs	Bmag0705 EBmag0705 Xmag705
Probe	EBmag705
Locus	EBmag0705 EBmag705 Bmag0705
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (TC)31 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.8
Type	PCR SSR
PCR primers	5' TCCTGTAGTCCTCTTTGTTTC 3' 5' TATATTACACATGGAGAGAGAGG 3' TCCTGTAGTCCTCTTTGTTTC TATATTACACATGGAGAGAGAGG
SSR size	155
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare