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GrainGenes Marker Report: EBmag757

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Probe	EBmag757
Locus	EBmag0757
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AG)31 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.68
Type	PCR SSR
PCR primers	5' GTGTCTTTTTCACTTCCTTTG 3' 5' TCTTCACTGTTGAGATGATGA 3'
SSR size	116
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare