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GrainGenes Marker Report: EBmag793

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Probe
EBmag793
Locus
EBmag0793
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (GT)13,(AG)36
Quality = 1: Single product, only weak stutter bands
Diversity Index (DI) = 0.69
Type
PCR
SSR
PCR primers
5' ATATATCAGCTCGGTCTCTCA 3'
5' AACATAGTAGAGGCGTAGGTG 3'
SSR size
177
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare