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GrainGenes Marker Report: GBM1001-5H

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Locus	GBM1001-5H
Type	Microsatellite
Map	Hv-Consensus2006-Stein-5H 181.3 Hv-DxR-Stein-5H 206.15
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, Stein Barley, OWB, Stein2006
Probe	GBM1001
Probe for Locus	GBM1001
Locus	GBM1001 GBM1001-5H
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Thiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
General Remarks	Primer sequences for this SSR are proprietary, those interested should contact Andreas Graner Clone HY04J22u
Type	SSR
PCR primers	available upon request to authors: graner@ipk-gatersleben.de
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	AL510334
DNA Library	IPK-HY
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)