GrainGenes Marker Report: GBM1002
Locus
GBM1002
Type
Microsatellite
Chromosome
1H
Map
Barley, B73xCPI-1H 90.5 Barley-ConsensusFHB-1H 111 Hordeum-Consensus2007-SSR-1H 101.5 Hv-Integrated2009-1H 103.2
[ Show Nearby Loci ]
Map Data
Barley, B73xCPI Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009
Species
Hordeum vulgare
Probe
GBM1002
Reference
Remark
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
[ Show all 6 ]
Probe
GBM1002
Locus
GBM1002 GBM1002-1H
Reference
Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Thiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remarks
Primer sequences for this SSR are proprietary, those interested should contact Andreas Graner Clone HW07P19V
Type
SSR
PCR primers
available upon request to authors: graner@ipk-gatersleben.de
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
AL505284
DNA Library
IPK-HW
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
GrainGenes Marker Report: GBM1002
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