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GrainGenes Marker Report: GBM1036-2H

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Locus	GBM1036-2H
Type	Microsatellite
Map	Hv-Consensus2006-Stein-2H 163.7 Hv-DxR-Stein-2H 173.04
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Map Data	Barley, Consensus 2006, Stein Barley, OWB, Stein2006
Probe	GBM1036
Probe for Locus	GBM1036
Locus	GBM1036 GBM1036-2H
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Thiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
General Remarks	Primer sequences for this SSR are proprietary, those interested should contact Andreas Graner Clone HW04F09u
Type	SSR EST
PCR primers	available upon request to authors: graner@ipk-gatersleben.de
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	AL501480
DNA Library	IPK-HW
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)