GrainGenes Marker Report: GBM1115
Locus
GBM1115
Type
Microsatellite
Chromosome
2H 7H
Map
Barley-ConsensusFHB-2H 49.13 Barley-ConsensusFHB-7H 77.8 Hordeum-Consensus2006-DArT-2H 58.8 Hordeum-Consensus2007-SSR-7H 81.85 Hv-Integrated2009-7H 79.5
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, DArT Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009
Species
Hordeum vulgare
Probe
GBM1115
Reference
Remark
Number of populations contributing to the placement of GBM1115 in the Barley, Consensus 2006 DArT maps = 1
[ Show all 7 ]
Probe
GBM1115
Locus
GBM1115 GBM1115-7H
Reference
Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks
Clone HI01L13T
Type
SSR
PCR primers
GTGCCGGTCCTTCATGTC
GCCTTCACGTAGTCCCAGAC
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
BQ462692
DNA Library
IPK-HI
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
GrainGenes Marker Report: GBM1115
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