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GrainGenes Marker Report: GBM1142

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Locus	GBM1142
Type	Microsatellite
Map	Hv-Integrated2009-1H 64.2
	[ Show Nearby Loci ]
Map Data	Barley, Integrated, Marcel 2009
Probe	GBM1142
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Oregon Wolfe Barleys
Probe	GBM1142
Locus	GBM1142 GBM1142-1H
Reference	Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks	Clone HP01H24w
PCR primers	AAAGCCAGTCGTAGACGGTG GAAGCCGTCGCAGTCGAT
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	BQ661852
DNA Library	IPK-HP
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)