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GrainGenes Marker Report: GBM1181

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Locus	GBM1181
Type	Microsatellite
Map	Hv-Integrated2009-2H 106
	[ Show Nearby Loci ]
Map Data	Barley, Integrated, Marcel 2009
Probe	GBM1181
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Oregon Wolfe Barleys
Probe	GBM1181
Locus	GBM1181 GBM1181-2H
Reference	Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks	Clone HV01B22T
PCR primers	GGAGAACCCAGTGGTGCTAA TCGACATTGGAGAACATGGA
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	BQ471142
DNA Library	IPK-HV
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)