GrainGenes Marker Report: GBM1232
Locus
GBM1232
Type
Microsatellite
Chromosome
2H
Map
Barley-ConsensusFHB-2H 73.03 Hordeum-Consensus2006-DArT-2H 77.4 Hordeum-Consensus2007-SSR-2H 72.16 Hv-Integrated2009-2H 64.8
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, DArT Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009
Species
Hordeum vulgare
Probe
GBM1232
Reference
Remark
Number of populations contributing to the placement of GBM1232 in the Barley, Consensus 2006 DArT maps = 1
[ Show all 7 ]
Probe
GBM1232
Locus
GBM1232 GBM1232-2H
Reference
Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks
Clone HH01F06u
Type
SSR
PCR primers
TTGGTTCTAGCCACACCTAAAA
GTTTTCGTCACACGTCCCTT
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
CB863094
DNA Library
IPK-HH
Source Species
Hordeum vulgare
Source Germplasm
Sloop (barley)
GrainGenes Marker Report: GBM1232
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