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GrainGenes Marker Report: GBM1323

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Locus	GBM1323
Type	Microsatellite
Chromosome	4H
Map	Barley, B73xCPI-4H 29.3 Barley-ConsensusFHB-4H 28.59 Hordeum-Consensus2007-SSR-4H 28.96 Hv-Integrated2009-4H 38.6
	[ Show Nearby Loci ]
Map Data	Barley, B73xCPI Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009
Species	Hordeum vulgare
Probe	GBM1323
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 6 ]
Probe	GBM1323
Locus	GBM1323 GBM1323-4H
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks	Clone HZ01J22r
Type	SSR
PCR primers	Forward GCTCTCCAGGGTTCGTTTC Reverse CACCGTCTTGCAGTTGAGAC GCTCTCCAGGGTTCGTTTC CACCGTCTTGCAGTTGAGAC
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	BQ469705
DNA Library	IPK-HZ
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)