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GrainGenes Marker Report: GBM1350

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Locus	GBM1350
Type	Microsatellite
Chromosome	4H
Map	Barley-ConsensusFHB-4H 88.27 Hordeum-Consensus2007-SSR-4H 78.23 Hv-Consensus2006-Marcel-4H 78.23 Hv-Integrated2009-4H 88.2 Hv-L94/Vada-2006-4H 53.98
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009 Barley, L94 x Vada, 2006
Species	Hordeum vulgare
Probe	GBM1350
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' [ Show all 7 ]
Probe	GBM1350
Locus	GBM1350 GBM1350-4H
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks	Primer sequences for this SSR to be released in the public domain soon (noted: September 2006). Those interested should contact Andreas Graner Clone HA12H19r
Type	SSR
PCR primers	Forward GTCCCTGCATCCTCTCCC Reverse CGTTATGTTGAAGCAGCCCT GTCCCTGCATCCTCTCCC CGTTATGTTGAAGCAGCCCT
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	BU978048
DNA Library	IPK-HA
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)