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GrainGenes Marker Report: GBM1413

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Locus
GBM1413
Type
Microsatellite
Chromosome
3H
Map
Barley-ConsensusFHB-3H54.89
Hordeum-Consensus2007-SSR-3H49.69
Hv-Integrated2009-3H67
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Map Data
Barley, Consensus 2007, SSR
Barley, Consensus 2023, FHB
Barley, Integrated, Marcel 2009
Species
Hordeum vulgare
Probe
GBM1413
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceVarshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remark
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
[ Show all 6 ]
Probe
GBM1413
Locus
GBM1413
GBM1413-3H
Reference
ReferenceHayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80.
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceVarshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks
Clone HV04M04u
Type
SSR
PCR primers
Forward GGGTGATTTCCCAGGTTTTT
Reverse TTGAGAAAACCACACCCACA
GGGTGATTTCCCAGGTTTTT
TTGAGAAAACCACACCCACA
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
BQ664760
DNA Library
IPK-HV
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)