GrainGenes Marker Report: GBM1424-3H
Locus
GBM1424-3H
Type
Microsatellite
Map
Hv-Consensus2006-Stein-3H 199.3 Hv-DxR-Stein-3H 210.29
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Stein Barley, OWB, Stein2006
Probe
GBM1424
Probe for Locus
GBM1424
Locus
GBM1424-3H
Reference
Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
General Remarks
Clone HI04N22r
PCR primers
CCTCTCCTCCTACCAAACCC
GAGGGAGAGGTTGTCCTCCT
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
BQ463333
DNA Library
IPK-HI
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
GrainGenes Marker Report: GBM1424-3H
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