GrainGenes Marker Report: GBM1452
Locus
GBM1452
Type
Microsatellite
Chromosome
4H
Map
Barley, B73xCPI-7H 84.3 Barley-ConsensusFHB-4H 57.31 Hordeum-Consensus2007-SSR-4H 54.87 Hv-Integrated2009-4H 70.1
[ Show Nearby Loci ]
Map Data
Barley, B73xCPI Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Integrated, Marcel 2009
Species
Hordeum vulgare
Probe
GBM1452
Reference
Remark
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
[ Show all 6 ]
Probe
GBM1452
Locus
GBM1452 GBM1452-4H
Reference
Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
Remarks
Clone HB17D02r
Type
SSR
PCR primers
GGAACCCATCTTACACCTGG
CCAAAGCAAAAAGTTCGGAG
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
BU971291
DNA Library
IPK-HB
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
GrainGenes Marker Report: GBM1452
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