GrainGenes Marker Report: GBS0014-3H
Locus
GBS0014-3H
Type
SNP
Map
Hv-Consensus2006-Stein-3H 110.8 Hv-DxR-Stein-3H 115.84
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Stein Barley, OWB, Stein2006
Probe
GBS14
Probe for Locus
GBS14
Locus
GBS0014 GBS0014-3H
Reference
Stein N et al. (2007) A 1000 loci transcript map of the barley genome - new anchoring points for integrative grass genomics Theoretical and Applied Genetics 114:823-839.
General Remarks
Clone HW05A07u
PCR primers
CTGTTAAGCCAACCCGCGT
GGTGATGAACCTCCATGGCT
Amplification Conditions
5 min at 94 deg C; 50 cycles with 30 sec at 94 deg C, 1 min at 65 deg C (touchdown of 1 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 40 cycles), 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
AL501682
DNA Library
IPK-HW
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
GrainGenes Marker Report: GBS0014-3H
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