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GrainGenes Marker Report: GBS0089

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Locus	GBS0089
Type	SNP
Map	Hv-Integrated2009-4H 143.4
	[ Show Nearby Loci ]
Map Data	Barley, Integrated, Marcel 2009
Probe	GBS89
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Steptoe x Morex
Probe for Locus	GBS89
Locus	GBS0089 GBS0089-4H
Reference	Stein N et al. (2007) A 1000 loci transcript map of the barley genome - new anchoring points for integrative grass genomics Theoretical and Applied Genetics 114:823-839.
General Remarks	Clone HW02F05T
PCR primers	GGTGTTGAGTGCTGGATGGA GCTGCAGTTTTGGCTGTGC
Amplification Conditions	5 min at 94 deg C; 50 cycles with 30 sec at 94 deg C, 1 min at 65 deg C (touchdown of 1 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 40 cycles), 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	AL503490
DNA Library	IPK-HW
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)