GrainGenes Marker Report: GBS0208-5H
Locus
GBS0208-5H
Type
SNP
Map
Hv-Consensus2006-Stein-5H 117.5 Hv-IxF-Stein-5H 81.08
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Stein Barley, IxF, Stein2006
Probe
GBS208
Probe for Locus
GBS208
Locus
GBS0208 GBS0208-5H
Reference
Stein N et al. (2007) A 1000 loci transcript map of the barley genome - new anchoring points for integrative grass genomics Theoretical and Applied Genetics 114:823-839.
General Remarks
Clone HW08E05u
PCR primers
AAGATCCAAATGCCAGCACG
GCTGATGACATGGAAGCAGTTT
Amplification Conditions
5 min at 94 deg C; 50 cycles with 30 sec at 94 deg C, 1 min at 65 deg C (touchdown of 1 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 40 cycles), 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
AL502637
DNA Library
IPK-HW
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
GrainGenes Marker Report: GBS0208-5H
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