GrainGenes Marker Report: GBS0553-7H
Locus
GBS0553-7H
Type
SNP
Map
Hv-Consensus2006-Stein-7H 26.7 Hv-SxM-Stein-7H 25.02
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Stein Barley, SxM, Stein2006
Probe
GBS553
Probe for Locus
GBS553
Locus
GBS0553 GBS0553-7H
Reference
Stein N et al. (2007) A 1000 loci transcript map of the barley genome - new anchoring points for integrative grass genomics Theoretical and Applied Genetics 114:823-839.
General Remarks
Clone HK06B06r
PCR primers
TTGCCAAGATGTACGACGCT
CCACAACCGGCGTAAAATAA
Amplification Conditions
5 min at 94 deg C; 50 cycles with 30 sec at 94 deg C, 1 min at 65 deg C (touchdown of 1 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 40 cycles), 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
AL500321
DNA Library
IPK-HK
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
GrainGenes Marker Report: GBS0553-7H
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