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GrainGenes Marker Report: GBS0875-4H

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Locus	GBS0875-4H
Type	SNP
Map	Hv-Consensus2006-Stein-4H 100.4 Hv-DxR-Stein-4H 101.4
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Map Data	Barley, Consensus 2006, Stein Barley, OWB, Stein2006
Probe	GBS875
Probe for Locus	GBS875
Locus	GBS0875 GBS0875-4H
Reference	Stein N et al. (2007) A 1000 loci transcript map of the barley genome - new anchoring points for integrative grass genomics Theoretical and Applied Genetics 114:823-839.
General Remarks	Clone HU08G10u
PCR primers	TCAGTTTTCGACTCCTACAATGC AACAGCTCTTGGCCAGCTC
Amplification Conditions	5 min at 94 deg C; 50 cycles with 30 sec at 94 deg C, 1 min at 65 deg C (touchdown of 1 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 40 cycles), 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	CA005903
DNA Library	IPK-HU
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)