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GrainGenes Marker Report: GBS554

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Probe	GBS554
Locus	GBS0554 GBS0554-1H
Reference	Stein N et al. (2007) A 1000 loci transcript map of the barley genome - new anchoring points for integrative grass genomics Theoretical and Applied Genetics 114:823-839.
Remarks	Clone HG01K10u
PCR primers	ATGGAGCCCCTCCCAACTAC GTAGACGTCCAGCACCTCGAT
Amplification Conditions	5 min at 94 deg C; 50 cycles with 30 sec at 94 deg C, 1 min at 65 deg C (touchdown of 1 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 40 cycles), 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	BQ660019
DNA Library	IPK-HG
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)