Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: GPW4043

[Submit comment/correction]

Probe	GPW4043
Locus	Xgpw4043-2B Sr47 gpw4043 gpw4043-2B
Reference	Klindworth DL et al. (2012) Introgression and Characterization of a Goatgrass Gene for a High Level of Resistance to Ug99 Stem Rust in Tetraploid Wheat G3: Genes, Genomes, Genetics 2:665-673. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stem Rust Resistance. Sr47 MAS Wheat. Marker Assisted Selection in Wheat.
Remarks	Motif (GA)16
Type	PCR SSR
PCR primers	Forward ACATATGCACGCACGCAC Reverse CATTGACACCCCTGACACTC 5'- ACATATGCACGCACGCAC -3' 5'- CATTGACACCCCTGACACTC -3'
SSR size	Expected 162
Amplification Conditions	Tm = 60C Denaturing step: 94C, 3 min. Touchdown step 1 (0.5C down each cycle until final annealing temperature): 94C, 30 sec; 65C, 30 sec; 72C, 2 min. Amplification step (30 cycles): 94C, 30 sec; 60C, 30 sec; 72C, 2 min. Extension step: 72C, 10 min
Linkage Group	2B
Source Species	Triticum aestivum