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GrainGenes Marker Report: H9/H10/H11

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Locus
H9/H10/H11
Type
Trait
Chromosome
1A
Chromosome Arm
1AS
Map
Wheat-Composite2004-1A27
[ Show Nearby Loci ]
Species
Triticum aestivum
Probe
CFA2153
Associated Gene
H10 (Triticum)
H11 (Triticum)
H9 (Triticum)
Gene Class
Reaction to Mayetiola destructor (Say) (Phytophaga destructor) (Say)
Reference
ReferenceLiu XM et al. (2005) H9, H10, and H11 compose a cluster of Hessian fly-resistance genes in the distal gene-rich region of wheat chromosome 1AS Theoretical and Applied Genetics 110:1473-1480.
Data Source
Matthews, David E.
Remark
Trait marker Xbarc263-1A, Xcfa2153-1A
Nearby marker on Composite map: Xbarc263, Xcfa2153
Position on Composite map estimated by GrainGenes.



Probe for Locus
CFA2153
Locus
Xcfa2153-1A
Xcfa2153-1A.1
Xcfa2153-1A.2
Xcfa2153
H9/H10/H11
Reference
ReferenceSourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = A
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (CT)27
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' TTGTGCATGATGGCTTCAAT 3'
Reverse 5' CCAATCCTAATGATCCGCTG 3'
SSR size
200
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
1A
Source Species
Triticum urartu