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GrainGenes Marker Report: HVARHGN

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Locus	HvARHGN
Chromosome	1H
Chromosome Arm	1HL
Probe	HVARHGN
Reference	Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remark	Assigned to a barley chromosome by PCR screening of wheat barley addition lines
Probe	HVARHGN
Locus	HvARHGN
Synonym	Synonym HAR35_15835 HAR35_35744 HAR35_38210
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remarks	Insert contains repeat (t)10
Type	SSR
PCR primers	Forward TCCATGGATGCAGAAGTAC Reverse CTCTTTCTAGTGTTCGTGGTC Left 5' TCTGCAAAGATTCCACCGGC Right 5' TCCAACCAGGGTGCATTTCC
SSR size	155
Amplification Conditions	High Stringency: 10 cycles of 1 min at 94 deg C; 0.5 min starting at 64 deg. C and decreasing 1deg C per cycle, 1 min at 72 deg C, followed by 30 cycles of 1 min at 94 deg C; 1 min at 55 deg C; 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	Z48360
Source Species	Hordeum vulgare