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GrainGenes Marker Report: HVDHN009

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Locus	HVDHN009
Type	Microsatellite
Map	Hordeum-Bins-5H 117
	[ Show Nearby Loci ]
Map Data	Barley, BinMap 2005
Probe	HVDHN9
Data Source	Kleinhofs, Andris
Remark	This marker is a Binlocus in the Hordeum-Bins maps, i.e. a marker mapped in other crosses and located to the Steptoe x Morex bin
Probe for Locus	HVDHN9
Locus	HVDHN009
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Becker J and Heun M (1995) Barley microsatellites: allele variation and mapping Plant Molecular Biology 27:835-845.
General Remarks	PCR size is expected size (kb) based on published sequence. mRNA for dehydrin-9. Insert contains repeat (TG)6.
Type	PCR SSR
PCR primers	5' CATGGACAAGATCAAGGAGAAG 3' 5' CCCATTATTTATCTGTAGGAACGC 3' Forward CATGGACAAGATCAAGGAGAAG Reverse CCCATTATTTATCTGTAGGAACGC
SSR size	0.128
Sequence	X15289
Source Gene Class	Dehydrin
Source Species	Hordeum vulgare