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GrainGenes Marker Report: HVHOTR1

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Locus	HVHOTR1
Type	Microsatellite
Chromosome	2H
Map	Barley-ConsensusFHB-2H 74.03 Hordeum-Consensus2007-SSR-2H 76.16 Hordeum-LxHs-2H 38
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, LxHs
Species	Hordeum spontaneum Hordeum vulgare
Probe	HVHOTR1
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (CAA)6 Polymorphic Information Content (PIC) = 0.12 Developing laboratory = SCRI (R. Waugh)
Probe	HVHOTR1
Locus	HVHOTR1
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (CAA)6 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.12
Type	PCR SSR
PCR primers	5' ATGAGCAGTCTTGTCTTAACC 3' 5' AGTTGGTCGCTAGATCTTATG 3' ATGAGCAGTCTTGTCTTAACC AGTTGGTCGCTAGATCTTATG Forward ATGAGCAGTCTTGTCTTAACC Reverse AGTTGGTCGCTAGATCTTATG
SSR size	165
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare