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GrainGenes Marker Report: HVM0009

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Locus	HVM0009
Type	Microsatellite
Map	Barley, B73xCPI-3H 42.1
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Map Data	Barley, B73xCPI
Probe	HVM9
Probe for Locus	HVM9
Locus	HVM9 HVM0009 HVM009
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Saghai Maroof MA et al. (1994) Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics. Proceedings of the National Academy of Sciences, USA 91:5466-5470.
General Remarks	Seed imbibition protein. Insert contains repeat (TCT)5.
Type	PCR SSR
PCR primers	5' CTTCGACACCATCACCCAG 3' 5' ACCAAAATCGCATCGAACAT 3' CTTCGACACCATCACCCAG ACCAAAATCGCATCGAACAT
SSR size	0.221
Amplification Conditions	PCR comprised one cycle of 94oC for 3 min, 55oC for 2 min, and 72oC for 1.5 min, followed by 30 cycles of 94oC for 1 min, 55oC for 2 min, and 72oC for 1.5 min.
Sequence	M77475
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.