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GrainGenes Marker Report: HVM06

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Locus	HVM06
Type	Microsatellite
Map	Hv-Integrated2009-5H 179.9
	[ Show Nearby Loci ]
Map Data	Barley, Integrated, Marcel 2009
Probe	HVM6
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Nure x Tremois
Probe for Locus	HVM6
Locus	HVM6 HVM0006 HVM006 HVM06
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
General Remarks	Insert contains repeat (GA)9.
Type	PCR SSR
PCR primers	5' CATGAATGAATGATTGGTTTTG 3' 5' CGCATCCGTATGTATGAGTAA 3' CATGAATGAATGATTGGTTTTG CGCATCCGTATGTATGAGTAA
SSR size	0.175
Amplification Conditions	The 'touchdown' PCR comprised 48 cycles of 94oC for 1 min denaturing and 72oC for 1 min extension. Annealing (30 s) temperatures were progressively decreased by 1oC every second cycle from 64oC to 55oC. Annealing conditions of 1 min at 55oC were maintained during the final 30 cycles. The reaction ended with a 5-min extension at 72oC.
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.