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GrainGenes Marker Report: HVM11

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Locus
HVM11
Type
Microsatellite
Synonym
Correct nameHVM011
SynonymHVM11
Chromosome
6H
Map
Hordeum-Consensus2005-SNP-6H67.146
Hordeum-QTLConsensus-Abiotic-6H67.15
Hordeum-QTLConsensus-Agronomic-6H67.15
Hordeum-StMo-SNP-6H58.07
[ Show Nearby Loci ]
QTL with Locus Marker
QFhs.HaTu-6H
Map Data
Barley, Abiotic QTL Consensus
Barley, Agronomic QTL Consensus
Barley, Consensus 2005, SNP
Barley, Steptoe x Morex, SNP
Probe
HVM11
ReferenceLiu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Reference
ReferenceRostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.



Probe
HVM11
Locus
HVM11
HVM11a
HVM11b
HVM011
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceLiu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
ReferenceSaghai Maroof MA et al. (1994) Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics. Proceedings of the National Academy of Sciences, USA 91:5466-5470.
Remarks
Glucose-regulated protein (GRP94 homologue, heat-shock protein 90).
Insert contains repeats (GGA)3, (GGA), and (GGA)2.
Type
PCR
SSR
PCR primers
5' CCGGTCGGTGCAGAAGAG 3'
5' AAATGAAAGCTAAATGGGCGATAT 3'
CCGGTCGGTGCAGAAGAG
AAATGAAAGCTAAATGGCGATAT
SSR size
0.181
Amplification Conditions
PCR comprised one cycle of 94oC for 3 min, 55oC for 2 min, and 72oC for 1.5 min, followed by 30 cycles of 94oC for 1 min, 55oC for 2 min, and 72oC for 1.5 min.
Sequence
X67960
Insert Enzyme
TaqI
Source Species
Hordeum vulgare
Source Germplasm
Old ZZ (barley)
DNA Origin
Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.