GrainGenes Marker Report: HVM22
Locus
HVM22
Type
Microsatellite
Synonym
Correct name HVM022 Synonym HVM22
Chromosome
6H
Map
Hordeum-Consensus2005-SNP-6H 71.558 Hordeum-QTLConsensus-Abiotic-6H 71.56 Hordeum-QTLConsensus-Agronomic-6H 71.56 Hordeum-StMo-SNP-6H 62.7
[ Show Nearby Loci ]
Map Data
Barley, Abiotic QTL Consensus Barley, Agronomic QTL Consensus Barley, Consensus 2005, SNP Barley, Steptoe x Morex, SNP
Probe
HVM22 
Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Reference
Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
Probe
HVM22
Locus
HVM22 HVM022 HVM22b HVM22a
Reference
Remarks
Insert contains repeat (AC)13.
Type
PCR SSR
PCR primers
5' TTTTGGGGGATGCCTACATA 3'
5' TTTCAAATGGTTGGATTGGA 3' TTTTGGGGGATGCCTACATA
TTTCAAATGGTTGGATTGGA
SSR size
0.167
Amplification Conditions
The 'touchdown' PCR comprised 48 cycles of 94oC for 1 min denaturing and 72oC for 1 min extension. Annealing (30 s) temperatures were progressively decreased by 1oC every second cycle from 64oC to 55oC. Annealing conditions of 1 min at 55oC were maintained during the final 30 cycles. The reaction ended with a 5-min extension at 72oC.
Insert Enzyme
TaqI
Source Species
Hordeum vulgare
Source Germplasm
Old ZZ (barley)
DNA Origin
Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.
GrainGenes Marker Report: HVM22
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