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GrainGenes Marker Report: HVM26

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Locus	HVM26
Type	Microsatellite
Synonym	Correct name HVM026 Synonym HVM26
Chromosome	2H
Chromosome Arm	2HS
Map	Barley-ConsensusFHB-2H 73.03 [ Show all 8 ]
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 8 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	HVM26 Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Li JZ et al. (2003) Development and genetic mapping of 127 new microsatellite markers in barley Theoretical and Applied Genetics 107:1021-1027.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (CA)11 Developing laboratory = VPISU (MA Saghai Maroof)
Probe	HVM26
Locus	HVM26 HVM026
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Remarks	Insert contains repeat (CA)11.
Type	PCR SSR
PCR primers	5' GGCTATCACATTTGGTACCATC 3' 5' GCATGTGTAGGTGTTGGTGG 3' GGCTATCACATTTGGTACCATC GCATGTGTAGGTGTTGGTGG
SSR size	0.206
Amplification Conditions	The 'touchdown' PCR comprised 38 cycles of 94oC for 1 min denaturing, and 72oC for 1 min extension. Annealing (30 sec) temperatures were progressively decreased by 1oC every second cycle from 69oC to 60oC, at which temperature they remained constant for the final 20 cycles. The reaction ended with a 5-min extension at 72oC.
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.