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GrainGenes Marker Report: HVM51

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Locus	HVM51
Type	Microsatellite
Chromosome	7H
Map	Hordeum-Lx41-7H 67.3
	[ Show Nearby Loci ]
Map Data	Barley, Lx41
Probe	HVM51 Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Remark	Assigned to chromosome based on wheat-barley addition line assays.
Probe	HVM51
Locus	HVM51 HVM0051
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Remarks	Insert contains repeat (GA)3, (GGGA)3, and (GA)8.
Type	PCR SSR
PCR primers	5' TCTAAATTACCTTCCCAGCCA 3' 5' AAAGCAGACATGTAGGAGGTCA 3' TCTAAATTACCTTCCCAGCCA AAAGCAGACATGTAGGAGGTCA
SSR size	0.151
Amplification Conditions	The 'touchdown' PCR comprised 48 cycles of 94oC for 1 min denaturing and 72oC for 1 min extension. Annealing (30 s) temperatures were progressively decreased by 1oC every second cycle from 64oC to 55oC. Annealing conditions of 1 min at 55oC were maintained during the final 30 cycles. The reaction ended with a 5-min extension at 72oC.
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.