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GrainGenes Marker Report: HVM60

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Locus	HVM60
Type	Microsatellite
Synonym	Correct name HVM060 Synonym HVM60
Chromosome	3H
Chromosome Arm	3HL
Map	Barley-consensus-2003-3H 75.4 [ Show all 19 ]
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003 [ Show all 19 ]
Species	Hordeum spontaneum Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	HVM60 Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117. [ Show all 6 ]
Data Source	Langridge, Peter J.
Remark	Number of populations contributing to the placement of HVM60 in the Barley, Consensus 2006 DArT maps = 2 [ Show all 7 ]
Probe	HVM60
Locus	HVM60 HVM0060 HVM060
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Remarks	Insert contains repeats (AG)11 and (GA)14.
Type	PCR SSR
PCR primers	5' CAATGATGCGGTGAACTTTG 3' 5' CCTCGGATCTATGGGTCCTT 3' CAATGATGCGGTGAACTTTG CCTCGGATCTATGGGTCCTT
SSR size	0.115
Amplification Conditions	The 'touchdown' PCR comprised 48 cycles of 94oC for 1 min denaturing and 72oC for 1 min extension. Annealing (30 s) temperatures were progressively decreased by 1oC every second cycle from 64oC to 55oC. Annealing conditions of 1 min at 55oC were maintained during the final 30 cycles. The reaction ended with a 5-min extension at 72oC.
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.