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GrainGenes Marker Report: HVM70

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Locus	HVM70
Type	Microsatellite
Synonym	Correct name HVM070
Chromosome	3H
Chromosome Arm	3HL
Map	Barley-consensus-2003-3H 118 Hordeum-LxHs-3H 174
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003 Barley, LxHs
Probe	HVM70 Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Data Source	Langridge, Peter J.
Probe	HVM70
Locus	HVM70 HVM0070 HVM70a HVM070
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
Remarks	Insert contains repeat (CA)8.
Type	PCR SSR
PCR primers	5' CCGCCGATGACCTTCTC 3' 5' ACCCACGACCTATGGCAC 3' CCGCCGATGACCTTCTC ACCCACGACCTATGGCAC
SSR size	0.154
Amplification Conditions	PCR comprised one cycle of 94oC for 3 min, 55oC for 2 min, and 72oC for 1.5 min, followed by 30 cycles of 94oC for 1 min, 55oC for 2 min, and 72oC for 1.5 min.
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.